KREMEN1 Knockout cell line(RKO)

KREMEN1 Knockout cell line(RKO) Order

Catalog#

YKO-H004

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

KREMEN1 Knockout cell line (RKO)

Morphology

Epithelial-like, adherent

Passage ratio

1:2~1:4

Species

Human

Gene Name

KREMEN1

Gene ID

83999

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90%DMEM+10%FBS

Target region

Knockout region: Exon 2 (based on transcript KREMEN1-202)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:RKO			STR Info (Cell bank) Cell Line:RKO
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	13		11	13
D13S317	8	11		8	11
D7S820	8	10		8	10
D16S539	13	13		12	13
VWA	16	22		16	22
TH01	6	10		6	10
AMEL	X	X		X	X
TPOX	11	11		11	11
CSF1PO	8	10		8	10

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the KREMEN1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the RKO cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, KREMEN1 gene knockout KREMEN1 Knockout cell line (RKO) will be expanded and cryopreserved.

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